Abstract: The electron beam butt welding experiments of CuCrZr and Inconel 625 tubes were carried out. The joint morphology, microstructure, chemical composition and mechanical properties of each sample were observed and analyzed. The test results show that the CuCrZr/Inconel 625 joint is in good shape when the beam offset distance is 0.6 and 0.8 mm, it presents a dumbbell shape with wide sides and narrow middle. With the increase of the beam offset distance of the CuCrZr side, the Cu content in the weld increases. The phase of CuCrZr/Inconel 625 tube joint is mainly spherical Ni-based solid solution and Cu solid solution as the matrix, which makes the weld have better comprehensive mechanical properties. When the beam offset distance is 0.8 mm, the maximum tensile strength of the sample reaches 304 MPa and breaks in the heat affected zone of CuCrZr side.