Abstract: Aiming at the camera calibration of seam tracking systems in welding robot,the camera model in Open Source Computer Vision Library(OpenCV) was discussed,a profound investigation of the camera calibration process and geometric model was performed.Specially,the radial distortion and tangential distortion had been taken into account.Then the Bouguet corner point's extraction algorithm was applied,and a camera calibration algorithm based on OpenCV was implemented.The results show that the algorithm gives full play of the functions in OpenCV library,and extracts the checkboard corners successfully,both the precision and computational efficiency are improved.Therefore,it can satisfy the requirements of real-time performance in camera calibration of vision navigation system.