Abstract: The electron beam welding test of Inconel 718 nickel-based superalloy and 304 stainless steel was carried out, and the microstructure and mechanical properties of the joint were analyzed. The results show that the middle of the weld zone is composed of fine equiaxed grains, and in the near-nickel side and near-steel fusion lines, there are some growth directions. The hardness of the joints is different among WZ 304 stainless and Inconel 718 and the handless of WZ is higher than that of Inconel 718 and 304 stainless steel. When the welding beam is 8 mA and the welding speed is 700 mm/min, the tensile strength of the joint is 722 MPa. Tensile studies showed that the fracture occurred at the weld zone. The fracture featured a typical ductile fracture, consisting of the dimple of the isoaxial shape.